Biochem/physiol Actions

Reversible: yes

Primary TargetAkt

Cell permeable: yes

General description

A cell-permeable, reversible, and selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition ﹤5 M) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50 = 2-5 M), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2, the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein. The solid form of this compound is also available.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Tsvetkov, A.S., et al. 2010. Proc. Natl. Acad. Sci. USA107, 16982.Thimmaiah, K.N., et al. 2005. J. Biol. Chem.280, 31924.

Packaging

Packaged under inert gas

2 mg in Glass bottle

Physical form

A 20 mM (2 mg/262 µL) solution of Akt Inhibitor X (Cat. No. 124020) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Warning

Toxicity: Standard Handling (A)